Thawing and Seeding Procedures
Cell Based Assay Procedures
If you have any questions, please send a message to
Please refer to the Time course data on the data sheet when you examine condition of measurement.
※ We attach data sheet with each product which is purchased.
What is the quantity of ABC Transporter Membranes/Vesicles products?
The quantity of all ABC Transporter Membranes/Vesicles products is 2.5mg protein/500 uL/vial.
When you follow our protocol, 50 Vesicular transport assays or 125 ATPase assays can be performed with one vial of ABC Transporter product.
For ATPase Assay
ATPase_assay.pdf
For Vesicular Transport Assay
VT assay protocol for RI.pdf
VT assay protocol for fluorescent_probe.pdf
For Vesicular Transport Assay and ATPase assay
Buffer preparation protocol.pdf
Technical Information
ABC Transporter Products
Protocol for our products
SLC Transporter Products
For Transiently Expressing Cell Lines
* please check the version of Data Sheet.
Data Sheet is ver. 2.0 or 3.0
Transiently Expressing cell lines protocol ver. 1.5 .pdf
Data Sheet is ver. 4.0
Transiently Expressing cell lines protocol ver.2.0 .pdf
ABC Transporter and Assay
ABC Transporters
The ATP-Binding Cassette (ABC) transporters are a family of membrane proteins, members of which are found in various living organisms from microbes to humans. These transporters have ATPase activity and carry many drugs, fats, sterols and other endogenous metabolites. Forty-seven kinds of ABC transporter have already been identified in the human genome, and approximately 11 kinds are recognized as important drug transporters that transfer materials from the inside of cells to the outside.
ABC transporters may influence the absorption, distribution, metabolism, and excretion (ADME) properties, as well as the efficacy and toxicity of candidate drugs. It is, therefore, important to examine the interaction of candidate drugs with these transporters to help identify drugs with high efficacy and low adverse effects.
ATPase Assay
ABC transporter activity is driven by the energy derived from hydrolysis of ATP.
The ATPase activity increases when a substrate interacts with an ABC transporter. The activity can be estimated by determining the concentration of inorganic phosphate resulting from ATP hydrolysis (ATPase assay). It is possible, therefore,to evaluate the interaction of a drug with an ABC transporter by means of ATPase assay without the need for radioisotope labeling of substrates.
Thus, ATPase assay can be employed as a convenient in vitro screening tool for ABC transporter substrates.
FAQ
How should I prepare buffer for Vesicular Transport assay and ATPase assay?
We provide “Buffer Preparation Protocol” on our web-site. You can find the composition of buffer and preparative procedure in our protocol.
For Vesicular Transport assay, we also have launched three types of Reagent Set for your convenience. By purchasing these Reagent Sets, you do not need copmplicated reagent preparation.
What is the storage condition for ABC Transporter Membranes/Vesicles products?
Please sore them at a temperature of -80 degrees C.
